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A validated ion pairing HPLC method for the determination of enoxacin and its metabolite oxo-enoxacin in plasma and urine
Summary
Type:
HPLC
Application #:
103210
Catalog#:
720002.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x125mm
Detection:
UV, 340nm
Conditions:
eluent: 860ml 0.1M citric acid, 140ml acetonitrile, 6.5ml tetrabutylammonium hydroxide (20% in water), and 4.5 g ammonium perchlorate
flow rate 1ml/min
Authors:
Goebel K-J
Source:
J Liquid Chromatogr 14 (1991) 733 - 751
Details
Legend: Fig. 3. Chromatograms of enoxacin (E), its oxo-metabolite (M) and ciprofloxacin (IS, int. std.) in urine, a) human blank urine, diluted 1:50 with water (1) and after solid phase cleanup (2), b) 25µg
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